Albumin test

 

INTRODUCTION

The albumin test measure the amount of albumin in patient blood sample. The albumin is a protein made by our liver. Albumin help keep fluid in blood stream mainly bilirubin and other organic fluid.

PRINCIPLE

Albumin is known for its ability to bind many types of organic compounds Including organic dye. when albumin bind with bromocresol it make bromocresol blue - green complex. The intensity of the blue - green complex is directly proportional to the concentration of albumin in the sample

Reaction :- 

Albumin + BCG ------------->BCG complex.

REQUIREMENT

• 3 test tubes
• Serum
• Albumin reagent
• Albumin standard
• Distilled water
• Colorimeter

PROCEDURE

1. Take three text tube and Mark than test , standard and blank.
2. Take 1ml of reagent in each tube.
3. Add 10ul of distilled water into black marked tube.
4. Add 10ul of standard into standard marked tube.
5. Add 10ul of serum into test marked tube.
6. Mix and incubate for 5 minutes at 20-25 °C.
7. After the 5 minute set the colorimeter filter at 578nm.
8. Than blank tube use for set colorimeter at zero.
9. Than standard marked tube mixture pour into cuvette and put the cuvette on tube holder and note the O.D of standard.
10. Than test marked tube mixture pour into  cuvette and put the cuvette on test tube holder and note the O.D of test.

CALCULATION

Albumin =   O.D of test × cons. Of standard

                  O.D of standard

NORMAL RANGE

3.5 - 5.5 g/dl

CLINICAL SIGNIFICANCE

Decrease in case

• Hepatitis.
• Cirhosis.
• Malnutrition.

Increase in case

• Dehydration.